CBL recombinant monoclonal antibody, clone R01-9G1
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More Files
- More Functions
Western Blot
Western blot analysis of CBL in Hela lysates using human CBL recombinant monoclonal antibody, clone R01-9G1 (Cat # RAB01529).
Immunocytochemistry
Immunocytochemistry analysis of CBL (green) in K562 using human CBL recombinant monoclonal antibody, clone R01-9G1 (Cat # RAB01529), and DAPI(blue).
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against synthetic peptide of human CBL.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against a synthetic peptide corresponding to human CBL
Theoretical MW (kDa)
Calculated MW: 100 k
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1:50-1:200)
Immunoprecipitation (1:20)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 50 mM Tris-Glycine, pH 7.4 (0.15 M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C for short term. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of CBL in Hela lysates using human CBL recombinant monoclonal antibody, clone R01-9G1 (Cat # RAB01529).Immunocytochemistry
Immunocytochemistry analysis of CBL (green) in K562 using human CBL recombinant monoclonal antibody, clone R01-9G1 (Cat # RAB01529), and DAPI(blue).Immunoprecipitation
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Gene Info — CBL
Entrez GeneID
867Protein Accession#
P22681Gene Name
CBL
Gene Alias
C-CBL, CBL2, RNF55
Gene Description
Cas-Br-M (murine) ecotropic retroviral transforming sequence
Omim ID
165360Gene Ontology
HyperlinkGene Summary
The cbl oncogene was first identified as part of a transforming retrovirus which induces mouse pre-B and pro-B cell lymphomas. As an adaptor protein for receptor protein-tyrosine kinases, it positively regulates receptor protein-tyrosine kinase ubiquitination in a manner dependent upon its variant SH2 and RING finger domains. Ubiquitination of receptor protein-tyrosine kinases terminates signaling by marking active receptors for degradation. [provided by RefSeq
Other Designations
oncogene CBL2
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Interactome
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Pathway
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Disease
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