ATF3 recombinant monoclonal antibody, clone R05-2E0
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More Files
- More Functions
Western Blot
Western blot analysis of ATF3 in MCF-7, U251, HL-60 lysates using ATF3 antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human ATF3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human ATF3.
Theoretical MW (kDa)
Calculated MW: 21 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine, pH 7.4, (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of ATF3 in MCF-7, U251, HL-60 lysates using ATF3 antibody.Immunoprecipitation
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Gene Info — ATF3
Entrez GeneID
467Protein Accession#
P18847Gene Name
ATF3
Gene Alias
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Gene Description
activating transcription factor 3
Omim ID
603148Gene Ontology
HyperlinkGene Summary
Activating transcription factor 3 is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. Multiple transcript variants encoding two different isoforms have been found for this gene. The longer isoform represses rather than activates transcription from promoters with ATF binding elements. The shorter isoform (deltaZip2) lacks the leucine zipper protein-dimerization motif and does not bind to DNA, and it stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. [provided by RefSeq
Other Designations
ATF3deltaZip2|ATF3deltaZip2c|ATF3deltaZip3|OTTHUMP00000034887|OTTHUMP00000034890
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Interactome
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Disease
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