WTAP recombinant monoclonal antibody, clone R09-9D8
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human WTAP.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against recombinant protein corresponding to human WTAP.
Theoretical MW (kDa)
Calculated MW: 44 kD
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoprecipitation
The optimal working dilution should be determined by the end user.Storage Buffer
In 50mM Tris-Glycine pH 7.4 (0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA)
Storage Instruction
Store at 4°C. For longer storage, aliquot and store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of WTAP in K562, Hela lysates using WTAP antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry analysis of paraffin-embedded Human tonsil using WTAP antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.Immunoprecipitation
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Gene Info — WTAP
Entrez GeneID
9589Protein Accession#
Q15007Gene Name
WTAP
Gene Alias
DKFZp686F20131, KIAA0105, MGC3925
Gene Description
Wilms tumor 1 associated protein
Omim ID
605442Gene Ontology
HyperlinkGene Summary
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq
Other Designations
OTTHUMP00000017522|OTTHUMP00000017523|PNAS-132|WT1-associated protein|Wilms' tumour 1-associating protein|putative pre-mRNA splicing regulator female-lethal(2D)
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