CD4 recombinant monoclonal antibody, clone CE9.1 (Clenoliximab)
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More Files
- More Functions
Western Blot
Western Blot analysis of Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 at 1.5 ug/mL before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen.
Immunohistochemistry
Immunohistochemistry analysis of Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) was used to stain samples at a concentration of 3 ug/mL.
Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.
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Specification
Product Description
Mouse recombinant monoclonal antibody raised against human CD4.
Antibody Species
Mouse
Immunogen
Original antibody is raised against Domain 1 of human CD4.
Reactivity
Human
Specificity
The antibody binds to human CD4 with a Kd of 1.0 nM and an ED50 of 0.2 ug/mL.
Form
Liquid
Purification
Protein A affinity purification
Isotype
IgG1, Lambda
Recommend Usage
Flow cytometry
Immunohistochemistry
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS with 0.02% Proclin 300
Storage Instruction
Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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Applications
Western Blot
Western Blot analysis of Human thyroid tumour samples were resolved on a 15% SDS PAGE gel and blots probed with the human IgG4-chimeric version of CE9.1 at 1.5 ug/mL before detection using an anti-human IgG4 secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for CD4 is 51kDa (arrowhead). Here some non-specific bands can also be seen.Immunohistochemistry
Immunohistochemistry analysis of Anti-CD4 staining of formaldehyde fixed paraffin embedded guinea pig skin tissue, at 40x magnification. The human IgG4-chimeric version of CE9.1 (Clenoliximab) was used to stain samples at a concentration of 3 ug/mL.Flow Cytometry
Flow-cytometry were stained with an isotype control at a concentration of 1 ug/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer. -
Gene Info — CD4
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Interactome
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Pathway
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Disease
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