FluoTag-X4 anti-GFP, clone 1H1/1B2 (ATTO 488)
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Specifications
Product Description
FluoTag-X4 anti-GFP two single-domain antibody (sdAb, also known as NanoAbTM) raised against two distinct epitopes on GFP and its most common derivatives.
Antibody Species
Camelid
Produced in
E. coli
Specificity
Recognizes GFP (green fluorescent protein) and common GFP derivatives like EGFP, mEGFP, Sirius, tSapphire, Cerulean, eCFP, mTurquoise, acGFP, Emerald, superecliptic pHluorin, paGFP, superfolder GFP, eYFP, mVenus and Citrine.
Form
Lyophilized
Conjugation
ATTO 488
Two fluorophores of FluoTag-X4 series are site-specifically coupled to each individual FluoTag molecule.Concentration
5 uM
Recommend Usage
Immunofluorescence (1:500)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from PBS pH 7.4 containing 2.5 uM fluorescently labeled clone 1H1 and 2.5 uM fluorescently labeled clone 1B2. Reconsitute with 200 uL 50% glycerol.
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
It is selected from the alpaca in vitro synthetic gene library, produced by phage display derived recombinant antibodies.
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Applications
Immunofluorescence
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