EGR2 polyclonal antibody
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More Files
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of EGR2.
Immunogen
A synthetic peptide corresponding to human EGR2.
Sequence
HGTAGPDRKPFPC
Host
Goat
Theoretical MW (kDa)
50.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western blot (0.03-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
EGR2 polyclonal antibody (Cat # PAB7314) (0.03 ug/mL) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
EGR2 polyclonal antibody (Cat # PAB7314, 3.8 ug/mL) staining of paraffin embedded human colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — EGR2
Entrez GeneID
1959Protein Accession#
NP_00.0390.2Gene Name
EGR2
Gene Alias
AT591, CMT1D, CMT4E, DKFZp686J1957, FLJ14547, KROX20
Gene Description
early growth response 2 (Krox-20 homolog, Drosophila)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a transcription factor with three tandem C2H2-type zinc fingers. Defects in this gene are associated with Charcot-Marie-Tooth disease type 1D (CMT1D), Charcot-Marie-Tooth disease type 4E (CMT4E), and with Dejerine-Sottas syndrome (DSS). Multiple transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq
Other Designations
KROX-20, Drosophila, homolog (early growth response-2)|Krox-20 homolog|OTTHUMP00000062133|early growth response 2 protein
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Interactome
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Disease
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Publication Reference
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Neuropathy-associated Egr2 mutants disrupt cooperative activation of myelin protein zero by Egr2 and Sox10.
LeBlanc SE, Ward RM, Svaren J.
Molecular and Cellular Biology 2007 May; 27(9):3521.
Application:EMSA, WB, Rat, S16 cells.
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Neuropathy-associated Egr2 mutants disrupt cooperative activation of myelin protein zero by Egr2 and Sox10.
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