APPL1 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
APPL1 polyclonal antibody (Cat # PAB7191) (0.5 ug/mL) staining of A) rat aortic smooth muscle cell lysate B) after infection with human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of APPL1.
Immunogen
A synthetic peptide corresponding to human APPL1.
Sequence
C-NRYSRLSKKRENDK
Host
Goat
Theoretical MW (kDa)
79.7
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:64000)
Western Blot (0.3-1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
APPL1 polyclonal antibody (Cat # PAB7191) (0.5 ug/mL) staining of A) rat aortic smooth muscle cell lysate B) after infection with human APPL1 adenovirus for 48hrs. Detected by chemiluminescence. Data kindly provided by Dr. L. Jia, Ann Arbor, USA.Enzyme-linked Immunoabsorbent Assay
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Gene Info — APPL1
Entrez GeneID
26060Protein Accession#
NP_036228.1Gene Name
APPL1
Gene Alias
APPL, DIP13alpha
Gene Description
adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
Omim ID
604299Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq
Other Designations
AKT2 interactor|adaptor protein containing pH domain, PTB domain and leucine zipper motif 1|signaling adaptor protein DIP13alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function.
Mao X, Kikani CK, Riojas RA, Langlais P, Wang L, Ramos FJ, Fang Q, Christ-Roberts CY, Hong JY, Kim RY, Liu F, Dong LQ.
Nature Cell Biology 2006 May; 8(5):516.
Application：IF, WB-Ce, WB-Ti, Human, Mouse, C2C12, HEK 293, HeLa, INS1, L6 cells, Mouse brain, fat, liver, kidney, heart, hepatocytes, spleen.
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APPL1 binds to adiponectin receptors and mediates adiponectin signalling and function.
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