ITGB5 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of ITGB5.
Immunogen
A synthetic peptide corresponding to human ITGB5.
Sequence
C-TFNKFNKSYNGTVD
Host
Goat
Theoretical MW (kDa)
88
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:16000)
Western Blot (0.1-0.3 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ITGB5 polyclonal antibody (Cat # PAB6744) (0.1 ug/mL) staining of human ovary lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ITGB5
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Interactome
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Pathway
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Disease
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Publication Reference
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Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha(v)beta5 integrin for binding but not for internalization.
Finnemann SC, Bonilha VL, Marmorstein AD, Rodriguez-Boulan E.
PNAS 1997 Nov; 94(24):12932.
Application:IF, IHC-Fr, WB-Ti, Rat, RPE cells.
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Phagocytosis of rod outer segments by retinal pigment epithelial cells requires alpha(v)beta5 integrin for binding but not for internalization.
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