ACADM polyclonal antibody
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More Files
- More Functions
Western Blot (Tissue lysate)
ACADM polyclonal antibody (Cat # PAB6264) staining (0.05 ug/mL) of human heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACADM polyclonal antibody (Cat # PAB6264) (3.8 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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Specifications
Product Description
Goat polyclonal antibody raised against synthetic peptide of ACADM.
Immunogen
A synthetic peptide corresponding to human ACADM.
Sequence
C-RLIVAREHIDKYKN
Host
Goat
Theoretical MW (kDa)
46.6, 47.0
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:32000)
Western blot (0.01-0.1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
ACADM polyclonal antibody (Cat # PAB6264) staining (0.05 ug/mL) of human heart lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ACADM polyclonal antibody (Cat # PAB6264) (3.8 ug/mL) staining of paraffin embedded human liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — ACADM
Entrez GeneID
34Protein Accession#
NP_000007.1Gene Name
ACADM
Gene Alias
ACAD1, FLJ18227, FLJ93013, FLJ99884, MCAD, MCADH
Gene Description
acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Gene Ontology
HyperlinkGene Summary
This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
OTTHUMP00000011345|medium-chain specific acyl-CoA dehydrogenase
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Interactomes
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Pathways
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Diseases
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Publication Reference
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Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes.
Powelka AM, Seth A, Virbasius JV, Kiskinis E, Nicoloro SM, Guilherme A, Tang X, Straubhaar J, Cherniack AD, Parker MG, Czech MP.
The Journal of Clinical Investigation 2005 Dec; 16(1):125.
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Suppression of oxidative metabolism and mitochondrial biogenesis by the transcriptional corepressor RIP140 in mouse adipocytes.
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