ACSS2 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against partial recombinant human ACSS2.
Immunogen
Recombinant protein corresponding to human ACSS2.
Sequence
PGETTQITYHQLLVQVCQFSNVLRKQGIQKGDRVAIYMPMIPELVVAMLACARIGALHSIVFAGFSSESLCERILDSSCSLLITTDAFYRGEKLVNLKELADEALQKCQEKGFPVRCCIVVKHLGRAELGMGDSTSQSPPIKRSCPDVQ
Host
Rabbit
Reactivity
Human, Mouse
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunofluorescence (1-4 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of Lane 1: NIH-3T3 cell lysate (mouse embryonic fibroblast cells) and Lane 2: NBT-II cell lysate (Wistar rat bladder tumor cells) with ACSS2 polyclonal antibody (Cat # PAB30675).Western Blot
Western Blot analysis of Lane 1: RT-4, Lane 2: U-251MG sp, Lane 3: human plasma (IgG/HSA depleted), Lane 4: human liver and Lane 5: human tonsil lysates with ACSS2 polyclonal antibody (Cat # PAB30675).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human duodenum with ACSS2 polyclonal antibody (Cat # PAB30675) shows moderate cytoplasmic and nuclear positivity in glandular cells.Immunofluorescence
Immunofluorescent staining of A-431 cells with ACSS2 polyclonal antibody (Cat # PAB30675) (Green) shows positivity in cytoplasm, vesicles and nucleus but excluded from the nucleoli. -
Gene Info — ACSS2
Entrez GeneID
55902Protein Accession#
Q9NR19Gene Name
ACSS2
Gene Alias
ACAS2, ACS, ACSA, AceCS, DKFZp762G026, dJ1161H23.1
Gene Description
acyl-CoA synthetase short-chain family member 2
Omim ID
605832Gene Ontology
HyperlinkGene Summary
This gene encodes a cytosolic enzyme that catalyzes the activation of acetate for use in lipid synthesis and energy generation. The protein acts as a monomer and produces acetyl-CoA from acetate in a reaction that requires ATP. Expression of this gene is regulated by sterol regulatory element-binding proteins, transcription factors that activate genes required for the synthesis of cholesterol and unsaturated fatty acids. Alternative splicing results in multiple transcript variants. [provided by RefSeq
Other Designations
OTTHUMP00000030712|OTTHUMP00000030713|OTTHUMP00000030714|OTTHUMP00000030715|OTTHUMP00000030716|acetate thiokinase|acetate-CoA ligase|acetyl-CoA synthetase|acetyl-Coenzyme A synthetase 2 (ADP forming)|acyl-activating enzyme|cytoplasmic acetyl-coenzyme A sy
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Interactome
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Pathway
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Disease
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