ATG7 polyclonal antibody
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More Files
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with ATG7 polyclonal antibody (Cat # PAB30240) shows moderate cytoplasmic positivity in trophoblastic cells at 1:50 - 1:200 dilution.
Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ATG7 polyclonal antibody (Cat # PAB30240) shows positivity in cytoplasm under 1-4 ug/mL concentration. Antibody staining is shown in green.
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Specification
Product Description
Rabbit polyclonal antibody raised against recombinant human ATG7.
Immunogen
Recombinant protein corresponding to amino acids of human ATG7.
Sequence
EAPKDIKGYYYNGDSAGLPARLTLEFSAFDMSAPTPARCCPAIGTLYNTNTLESFKTADKKLLLEQAANEIWESIKSGTALENPVLLNKFLLLTFADLKKYHFYYWFCYPALCLPESLPLIQGPVGLDQRFSLKQIEAL
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Isotype
IgG
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:200)
Immunofluorescence (1-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human placenta with ATG7 polyclonal antibody (Cat # PAB30240) shows moderate cytoplasmic positivity in trophoblastic cells at 1:50 - 1:200 dilution.Immunofluorescence
Immunofluorescent staining of human cell line A-431 with ATG7 polyclonal antibody (Cat # PAB30240) shows positivity in cytoplasm under 1-4 ug/mL concentration. Antibody staining is shown in green. -
Gene Info — ATG7
Entrez GeneID
10533Protein Accession#
O95352Gene Name
ATG7
Gene Alias
APG7-LIKE, APG7L, DKFZp434N0735, GSA7
Gene Description
ATG7 autophagy related 7 homolog (S. cerevisiae)
Omim ID
608760Gene Ontology
HyperlinkGene Summary
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq
Other Designations
APG7 autophagy 7-like|ubiquitin activating enzyme E1-like protein
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Interactome
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Pathway
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Disease
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