ADD1/ADD2 polyclonal antibody
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More Files
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ADD1/ADD2.
Immunogen
A synthetic peptide corresponding to human ADD1/ADD2.
Host
Rabbit
Theoretical MW (kDa)
80
Reactivity
Human, Mouse, Rat
Specificity
ADD1/ADD2 polyclonal antibody detects endogenous levels of ADD1/ADD2 protein.
Form
Liquid
Purification
Antigen affinity purification
Concentration
1 mg/mL
Recommend Usage
Western Blot (1:500-1:1000)
Immunohistochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunoprecipitor (1:50-1:200)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (0.05% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of ADD1/ADD2 polyclonal antibody (Cat # PAB27016) in extracts from HeLa cells.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of ADD1/ADD2 polyclonal antibody (Cat # PAB27016) in paraffin-embedded human brain tissue.Immunofluorescence
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — ADD1
Entrez GeneID
118Protein Accession#
P35611 (Gene ID : 118);P35612 (Gene ID : 119)Gene Name
ADD1
Gene Alias
ADDA, MGC3339, MGC44427
Gene Description
adducin 1 (alpha)
Gene Ontology
HyperlinkGene Summary
Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described. [provided by RefSeq
Other Designations
OTTHUMP00000151164|OTTHUMP00000151165|erythrocyte adducin alpha subunit
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Gene Info — ADD2
Entrez GeneID
119Protein Accession#
P35611 (Gene ID : 118);P35612 (Gene ID : 119)Gene Name
ADD2
Gene Alias
ADDB
Gene Description
adducin 2 (beta)
Omim ID
102681Gene Ontology
HyperlinkGene Summary
Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known. [provided by RefSeq
Other Designations
Adducin-2 (beta)|adducin 2|beta adducin
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