ATG13 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of 293T with ATG13 polyclonal antibody (Cat # PAB19949) at 1 ug/mL dilution. Lane 1: kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13; Lane 2: 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13).
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of ATG13.
Immunogen
A synthetic peptide corresponding to residues surrounding S318 of human ATG13.
Host
Rabbit
Theoretical MW (kDa)
56.6
Reactivity
Human
Specificity
A BLAST analysis was used to suggest cross reactivity with ATG13 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Form
Liquid
Purification
Immunoaffinity purification
Recommend Usage
ELISA (1:220000-1:250000)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293T with ATG13 polyclonal antibody (Cat # PAB19949) at 1 ug/mL dilution. Lane 1: kinase-dead hypophosphorylated Ulk1-K46A mutant + ATG13; Lane 2: 293T cells engineered to coexpress Ulk1 and Atg13 (Ulk1 + Atg13).Enzyme-linked Immunoabsorbent Assay
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Gene Info — ATG13
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