CNN2 polyclonal antibody
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More Files
- More Functions
Western Blot (Cell lysate)
CNN2 polyclonal antibody (Cat # PAB18723) (0.03 ug/mL) staining of HepG2 lysates (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of CNN2.
Immunogen
A synthetic peptide corresponding to amino acids near C-terminus of human CNN2.
Sequence
C-DPGEVPEYPPYYQEE
Host
Goat
Theoretical MW (kDa)
35
Reactivity
Human
Specificity
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with CCN3.
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
ELISA (1:32000)
Western Blot (0.03-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
CNN2 polyclonal antibody (Cat # PAB18723) (0.03 ug/mL) staining of HepG2 lysates (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.Enzyme-linked Immunoabsorbent Assay
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Gene Info — CNN2
Entrez GeneID
1265Protein Accession#
NP_004359.1;NP_958434.1Gene Name
CNN2
Gene Alias
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Gene Description
calponin 2
Omim ID
602373Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene, which can bind actin, calmodulin, troponin C, and tropomyosin, may function in the structural organization of actin filaments. The encoded protein could play a role in smooth muscle contraction and cell adhesion. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
calponin H2, smooth muscle|neutral calponin
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