BNIP3 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of BNIP3 (BH3 Domain Specific).
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 215-252 of human BNIP3.
Host
Rabbit
Reactivity
Human, Mouse
Specificity
BH3 Domain Specific.
Form
Liquid
Preparation Method
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Recommend Usage
ELISA
Immunofluorescence (1:50-100)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide).
Storage Instruction
Store at 4°C for up to 2 weeks. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with BNIP3 polyclonal antibody (Cat # PAB1863) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Immunofluorescence
Fluorescent confocal image of HepG2 cells stained with BNIP3 (BH3 Domain Specific) antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with BNIP3 polyclonal antibody (Cat # PAB1863) (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/mL, 5 min). BNIP3 immunoreactivity is localized to the cytoplasm of HepG2 cells.Immunofluorescence
Freshly isolated mouse hepatocytes plated on coverslips (2 X105 cells / 22-mm glass coverslip) were cultured under normoxic conditions for 6 hr. The cells were then fixed in 2% paraformaldehyde in PBS for 1 hr, and processed for confocal immunofluorescence (red: F-actin, blue: ATP-synthase, green: BNIP3). Fluorescence labeling of BNIP3 accomplished with BNIP3 polyclonal antibody (Cat # PAB1863). Data courtesy of Ruben Zamora, University of Pittsburgh.Enzyme-linked Immunoabsorbent Assay
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Gene Info — BNIP3
Entrez GeneID
664Protein Accession#
Q12983Gene Name
BNIP3
Gene Alias
NIP3
Gene Description
BCL2/adenovirus E1B 19kDa interacting protein 3
Omim ID
603293Gene Ontology
HyperlinkGene Summary
This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. This gene contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq
Other Designations
BCL2/adenovirus E1B 19kD-interacting protein 3|OTTHUMP00000020752
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Interactome
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Publication Reference
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Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress.
Metukuri MR, Beer-Stolz D, Namas RA, Dhupar R, Torres A, Loughran PA, Jefferson BS, Tsung A, Billiar TR, Vodovotz Y, Zamora R.
American Journal of Physiology. Gastrointestinal and Liver Physiology 2009 Jan; 296(3):G499.
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Expression and subcellular localization of BNIP3 in hypoxic hepatocytes and liver stress.
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