SNX1 polyclonal antibody
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Specification
Product Description
Goat polyclonal antibody raised against synthetic peptide of SNX1.
Immunogen
A synthetic peptide corresponding to amino acids at internal region of human SNX1.
Sequence
C-TNSSKPQPTYEELE
Host
Goat
Theoretical MW (kDa)
59.1, 51.8, 53.3
Reactivity
Human
Form
Liquid
Purification
Antigen affinity purification
Concentration
0.5 mg/mL
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)(3-5 ug/mL)
ELISA (1:16000)
Western Blot (0.05-0.1 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
SNX1 polyclonal antibody (Cat # PAB17862) (1 ug/mL) staining of HeLa lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human uterus with SNX1 polyclonal antibody (Cat # PAB17862) (3.8 ug/mL). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Enzyme-linked Immunoabsorbent Assay
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Gene Info — SNX1
Entrez GeneID
6642Protein Accession#
NP_003090.2;NP_690039.1Gene Name
SNX1
Gene Alias
HsT17379, MGC8664, SNX1A, Vps5
Gene Description
sorting nexin 1
Omim ID
601272Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq
Other Designations
sorting nexin 1A
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