NOS1 polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of NOS1.
Immunogen
A synthetic peptide corresponding to amino acids at C-terminus of human NOS1.
Host
Rabbit
Theoretical MW (kDa)
160
Reactivity
Human, Rat
Specificity
The synthetic peptide of this antibody is identical to the related rat sequence.
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Western Blot (1 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.9 mg NaCl, 0.2 mg Na2HPO4 (5 mg BSA, 0.05 mg sodium azide, 0.05 mg Thimerosal)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 200 uL of deionized water and concentration will be 500 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide and thimerosal: POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Tissue lysate)
Immunohistochemical staining in paraffin embedded rat brain with NOS1 polyclonal antibody (Cat # PAB17214).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Gene Info — NOS1
Entrez GeneID
4842Gene Name
NOS1
Gene Alias
IHPS1, NOS, nNOS
Gene Description
nitric oxide synthase 1 (neuronal)
Gene Ontology
HyperlinkGene Summary
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. This gene encodes a nitric oxide synthase which is highly expressed in skeletal muscle. Genetic variations in this gene are associated with infantile hypertrophic pyloric stenosis type 1
Other Designations
neuronal nitric oxide synthase
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Interactome
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Pathway
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Disease
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Publication Reference
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A novel, testis-specific mRNA transcript encoding an NH2-terminal truncated nitric-oxide synthase.
Wang Y, Goligorsky MS, Lin M, Wilcox JN, Marsden PA.
The Journal of Biological Chemistry 1997 Apr; 272(17):11392.
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Nitric oxide synthase complexed with dystrophin and absent from skeletal muscle sarcolemma in Duchenne muscular dystrophy.
Brenman JE, Chao DS, Xia H, Aldape K, Bredt DS.
Cell 1995 Sep; 82(5):743.
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A novel, testis-specific mRNA transcript encoding an NH2-terminal truncated nitric-oxide synthase.
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