DNMT3A polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of DNMT3A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to amino acids 107-121 of human DNMT3A.
Host
Rabbit
Reactivity
Human
Form
Liquid
Recommend Usage
ELISA (1:50)
Western Blot (1:2000)
Immunoprecipitation (2 ug/IP)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide, 0.05% proclin 300)
Storage Instruction
Store at -20°C. For long term storage store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Human embryonic kidney cells (HEK293T) were transiently transfected with an expression vector for GAL4-tagged DNMT3A.
Whole cell extracts were immunoprecipitated with 2 ug of DNMT3A polyclonal antibody (Cat # PAB14131).
The presence of GAL4-DNMT3A in the non-treated cell extracts and in the immunoprecipitates was demonstrated by western blot analysis with anti-GAL4 antibody (Lane 1 and 2 respectively).
Alternatively, GAL4-DNMT3A was immunoprecipitated and western blot analysis was performed with DNMT3A polyclonal antibody (diluted 1 : 2,000) (Lane 3).Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
ELISA was performed using a serial dilution of DNMT3A polyclonal antibody (Cat # PAB14131), crude serum and Flow Through in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 400. -
Gene Info — DNMT3A
Entrez GeneID
1788Protein Accession#
Q9Y6K1Gene Name
DNMT3A
Gene Alias
DNMT3A2, M.HsaIIIA
Gene Description
DNA (cytosine-5-)-methyltransferase 3 alpha
Omim ID
602769Gene Ontology
HyperlinkGene Summary
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
DNA MTase HsaIIIA|DNA cytosine methyltransferase 3 alpha|DNA cytosine methyltransferase 3A2|DNA methyltransferase HsaIIIA|OTTHUMP00000120005|OTTHUMP00000120006
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