JUN polyclonal antibody
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Specification
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of JUN.
Immunogen
A synthetic peptide corresponding to N-terminus of human JUN.
Host
Rabbit
Theoretical MW (kDa)
48
Reactivity
Human, Mouse, Rat
Specificity
This antibody recognizes ~48 KDa of human JUN.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
Western Blot (0.1-1 ug/mL)
ELISA (0.01-0.1 ug/mL)
Immunoprecipitation (2-5 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In TBS, pH 7.2 (BSA, 10% Proclin300)
Storage Instruction
Store at 4°C. For long term storage store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing. -
Applications
Western Blot (Cell lysate)
The cell lysate derived from NIH/3T3 cells was immunoprobed by JUN polyclonal antibody (Cat # PAB12669) at 1 : 500. An immunoreactive band is observed around ~48kDa (Lane 1). This band is blocked by pre-incubation with immunizing peptide (Lane 2).Immunohistochemistry
Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Gene Info — JUN
Entrez GeneID
3725Gene Name
JUN
Gene Alias
AP-1, AP1, c-Jun
Gene Description
jun oncogene
Omim ID
165160Gene Ontology
HyperlinkGene Summary
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq
Other Designations
Jun activation domain binding protein|OTTHUMP00000010036|activator protein 1|enhancer-binding protein AP1|v-jun avian sarcoma virus 17 oncogene homolog|v-jun sarcoma virus 17 oncogene homolog
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Interactome
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Pathway
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Disease
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Publication Reference
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A reinvestigation of the multisite phosphorylation of the transcription factor c-Jun.
Morton S, Davis RJ, McLaren A, Cohen P.
The EMBO Journal 2003 Aug; 22(15):3876.
Application:WB-Ce, Mouse, RAW 264.7 cells, Fibroblasts.
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A reinvestigation of the multisite phosphorylation of the transcription factor c-Jun.
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