SARS-CoV N polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using SARS-CoV N polyclonal antibody (Cat # PAB11373) shows detection of a 46- KDa band corresponding to the protein.
Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose.
The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h.
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Specifications
Product Description
Rabbit polyclonal antibody raised against full length recombinant SARS N.
Immunogen
Recombinant protein corresponding to full length SARS N (Nucleocapsid protein).
Host
Rabbit
Reactivity
SARS-CoV
Specificity
This protein A purified antibody is directed against SARS Coronavirus Nucleocapsid (N) protein.
Form
Lyophilized
Purification
The product was purified from monospecific antiserum by protein A affinity purification.
Recommend Usage
ELISA (1:10000-1:50000)
Flow Cytometry
Immunofluorescence
Immunohistochemistry (1:100-1:6000)
Western Blot (1:2000-1:10000)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide).
Storage Instruction
Store at 4°C on dry atmosphere prior to restoration.
After reconstitution with 100 uL of deionized water (or equivalent), aliquot and store at -20°C or lower. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using SARS-CoV N polyclonal antibody (Cat # PAB11373) shows detection of a 46- KDa band corresponding to the protein.
Approx. 100 ng of protein was loaded for SDS-PAGE and transferred onto nitrocellulose.
The blot was incubated with a 1:5,000 dilution of the antibody at room temperature for 1 h.Immunohistochemistry
Immunofluorescence
Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
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Gene Info — N
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Publication Reference
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Expression and purification of SARS coronavirus proteins using SUMO-fusions.
Zuo X, Mattern MR, Tan R, Li S, Hall J, Sterner DE, Shoo J, Tran H, Lim P, Sarafianos SG, Kazi L, Navas-Martin S, Weiss SR, Butt TR.
Protein Expression and Purification 2005 Jul; 42(1):100.
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Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.
Zuo X, Li S, Hall J, Mattern MR, Tran H, Shoo J, Tan R, Weiss SR, Butt TR.
Journal of Structural and Functional Genomics 2005 Jan; 6(2-3):103.
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SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.
Malakhov MP, Mattern MR, Malakhova OA, Drinker M, Weeks SD, Butt TR.
Journal of Structural and Functional Genomics 2004 Jan; 5(1-2):75.
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Expression and purification of SARS coronavirus proteins using SUMO-fusions.
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