SARS-CoV nsp8 polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Immunoprecipitation followed by western blotting using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) shows a predominant band at 21.8 KDa corresponding to full length SARS protein.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
Immunofluorescence
Immunofluorescence Microscopy using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) 6 hrs post infection of Vero-E6 cells.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.
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Specifications
Product Description
Rabbit polyclonal antibody raised against full length recombinant SARS-CoV nonstructural protein 8 (nsp8).
Immunogen
Recombinant protein corresponding to full length SARS-CoV nsp8.
Host
Rabbit
Reactivity
SARS-CoV
Specificity
This antibody is directed against SARS-Coronavirus nsp8 protein.
Form
Liquid
Purity
The product is neat antiserum.
Recommend Usage
Immunoelectron Microscopy (1:100)
Immunofluorescence (1:300)
Immunoprecipitation (1:60)
Western Blot (1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In antiserum (0.01% sodium azide).
Storage Instruction
Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage.
Aliquot to avoid repeated freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Immunoprecipitation followed by western blotting using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) shows a predominant band at 21.8 KDa corresponding to full length SARS protein.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.Immunofluorescence
Immunofluorescence Microscopy using SARS-CoV nsp8 polyclonal antibody (Cat # PAB11372) 6 hrs post infection of Vero-E6 cells.
Personal Communication, Eric Snijder, Leiden University Medical Center, Leiden, Netherlands.Immunoprecipitation
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Gene Info — orf1ab
Entrez GeneID
1489680Protein Accession#
P0C6U8; 30124074Gene Name
orf1ab
Gene Alias
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Gene Description
orf1ab polyprotein (pp1ab)
Gene Ontology
HyperlinkOther Designations
orf1a polyprotein (pp1a)
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Publication Reference
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Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.
Snijder EJ, van der Meer Y, Zevenhoven-Dobbe J, Onderwater JJ, van der Meulen J, Koerten HK, Mommaas AM.
Journal of Virology 2006 Jun; 80(12):5927.
Application：IEM, IF, Monkey, Vero cells.
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Identification and characterization of severe acute respiratory syndrome coronavirus replicase proteins.
Prentice E, McAuliffe J, Lu X, Subbarao K, Denison MR.
Journal of Virology 2004 Sep; 78(18):9977.
Application：IF, WB, Monkey, Vero cells.
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Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage.
Snijder EJ, Bredenbeek PJ, Dobbe JC, Thiel V, Ziebuhr J, Poon LL, Guan Y, Rozanov M, Spaan WJ, Gorbalenya AE.
Journal of Molecular Biology 2003 Aug; 331(5):991.
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Ultrastructure and origin of membrane vesicles associated with the severe acute respiratory syndrome coronavirus replication complex.
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