PCNA polyclonal antibody
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More Files
- More Functions
Western Blot (Recombinant protein)
Western blot using PCNA polyclonal antibody (Cat # PAB10179) shows detection of a predominant bandat ~36 KDa corresponding to PCNA (arrowhead lane 2).
Peptide competition effectively eliminates specific band reactivity (Lane 1).
For peptide competition, the antibody waspre-incubated with 100 ug (excess) of the peptide for 2h at 37°C followed by overnight incubation at 4°C, followed by abrief centrifugation and then reaction with the membrane.
Each lane contains a lysate from 105 cells from the marine dinoflagellate Prorocentrum minimum.
Cells were separatedby SDS-PAGE and transferred onto nitrocellulose using standard techniques.
After blocking the membrane was probed with the primary antibody diluted to 1:1000.
Incubation was followed by washes and reaction with HRPGt-a-Rabbit IgG [H&L] MX.
Detection was by ECL using a 30 sec exposure time.
Personnel Communication. S. Lin,U.Conn.
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Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of PCNA.
Immunogen
A synthetic peptide corresponding to amino acids 55-71 of Isochrysis galbana PCNA.
Host
Rabbit
Specificity
This antibody would react with PCNA from the following alga sources: Prorocentrum minimum, Tetraselmis chui, Pleurochrysis carterae and Isochrysis galbana. Expect partial reactivity with PCNA from other alga sources as well as carrot, tobacco, peas, soybean and rice based on partial protein sequence homology.
Form
Liquid
Quality Control Testing
Antibody Reactive Against Synthetic Peptide.
Recommend Usage
ELISA (1:10000-1:50000)
Western Blot (1:200-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Recombinant protein)
Western blot using PCNA polyclonal antibody (Cat # PAB10179) shows detection of a predominant bandat ~36 KDa corresponding to PCNA (arrowhead lane 2).
Peptide competition effectively eliminates specific band reactivity (Lane 1).
For peptide competition, the antibody waspre-incubated with 100 ug (excess) of the peptide for 2h at 37°C followed by overnight incubation at 4°C, followed by abrief centrifugation and then reaction with the membrane.
Each lane contains a lysate from 105 cells from the marine dinoflagellate Prorocentrum minimum.
Cells were separatedby SDS-PAGE and transferred onto nitrocellulose using standard techniques.
After blocking the membrane was probed with the primary antibody diluted to 1:1000.
Incubation was followed by washes and reaction with HRPGt-a-Rabbit IgG [H&L] MX.
Detection was by ECL using a 30 sec exposure time.
Personnel Communication. S. Lin,U.Conn.Immunoprecipitation
Enzyme-linked Immunoabsorbent Assay
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Publication Reference
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Identification and preliminary characterization of PCNA gene in the marine phytoplankton Dunaliella tertiolecta and Isochrysis galbana.
Lin S, Carpenter EJ.
Molecular Marine Biology and Biotechnology 1998 Mar; 7(1):62.
Application：IF, Plant, Dunaliella tertiolecta.
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Identification and preliminary characterization of PCNA gene in the marine phytoplankton Dunaliella tertiolecta and Isochrysis galbana.
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