TNFSF11 (Human) Recombinant Protein
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Specification
Product Description
Human TNFSF11 (O14788-2, Gly63-Asp244) partial recombinant protein expressed in HEK293 cells.
Sequence
Gly63-Asp244
Host
Human
Theoretical MW (kDa)
20.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 90% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 13.6 ng/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.030 nM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human RANKL is greater than 90% as determined by SEC-HPLC.
Tris-Bis PAGE
Human RANKL on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human RANKL, No Tag at 2 ug/mL (100 uL/well) on the plate. Dose response curve for Human TNFRSF11A, hFc Tag with the EC50 of 13.6 ng/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human TNFRSF11B, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human RANKL, No Tag with an affinity constant of 0.030 nM as determined in SPR assay (Biacore T200). -
Gene Info — TNFSF11
Entrez GeneID
8600Protein Accession#
O14788-2Gene Name
TNFSF11
Gene Alias
CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf
Gene Description
tumor necrosis factor (ligand) superfamily, member 11
Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell-dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. Two alternatively spliced transcript variants have been found. [provided by RefSeq
Other Designations
OTTHUMP00000178585|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of nuclear factor kappa B ligand|tumor necrosis factor ligand superfamily, member 11
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