LIFR (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human LIF at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.12 ug/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
SEC-HPLC
The purity of Human LIF R/CD118 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human LIF R/CD118 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. You may also be interested in Human LIF R/CD118 Protein (LIF-HM20R) From $282.00 Human LIF Protein (LIF-HM001) From $394.00 Human LIF Protein (LIF-HE001) From $300.00 Human MUC18/CD146 Protein (MUC-HM118) From $375.00 View more associated products Recently Viewed Products
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Specification
Product Description
Human LIFR (P42702-1, Gln45-Ser833) partial recombinant protein with His tag at C-Terminus expressed in HEK293 cells.
Sequence
Gln45-Ser833
Host
Human
Theoretical MW (kDa)
90.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 0.12 ug/mL, messured by ELISA at 2 ug/mL. The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human LIF R/CD118 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human LIF R/CD118 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. You may also be interested in Human LIF R/CD118 Protein (LIF-HM20R) From $282.00 Human LIF Protein (LIF-HM001) From $394.00 Human LIF Protein (LIF-HE001) From $300.00 Human MUC18/CD146 Protein (MUC-HM118) From $375.00 View more associated products Recently Viewed Products
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human LIF at 2 ug/mL (100 uL/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.12 ug/mL determined by ELISA (QC Test).Functional Study
SDS-PAGE
Surface Plasmon Resonance
Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). -
Gene Info — LIFR
Entrez GeneID
3977Protein Accession#
P42702-1Gene Name
LIFR
Gene Alias
CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS
Gene Description
leukemia inhibitory factor receptor alpha
Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit, gp130, to form a receptor complex that mediates the action of the leukemia inhibitory factor, a polyfunctional cytokine that is involved in cellular differentiation, proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
CD118 antigen|leukemia inhibitory factor receptor
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Interactome
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Pathway
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