CD8A (Human) Recombinant Protein
* The price is valid only in USA. Please select country.
-
More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-CD8 Antibody, hFc Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 4.8 ng/mL determined by ELISA.
Tris-Bis PAGE
Biotinylated Human CD8 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%
-
Specification
Product Description
Human CD8A (P01732-1, Ser22-Asp182) partial recombinant protein with His-Avi tag at C-Terminus expressed in HEK293 cells.
Sequence
Ser22-Asp182
Host
Human
Theoretical MW (kDa)
20.5
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 4.8 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
Tris-Bis PAGE
Biotinylated Human CD8 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
-
Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Anti-CD8 Antibody, hFc Tag at 0.5 ug/mL (100 uL/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 4.8 ng/mL determined by ELISA.Functional Study
SDS-PAGE
-
Gene Info — CD8A
Entrez GeneID
925Protein Accession#
P01732-1Gene Name
CD8A
Gene Alias
CD8, Leu2, MAL, p32
Gene Description
CD8a molecule
Gene Ontology
HyperlinkGene Summary
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain isoforms. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
CD8 antigen alpha polypeptide|CD8 antigen, alpha polypeptide (p32)|Leu2 T-lymphocyte antigen|OKT8 T-cell antigen|T cell co-receptor|T-cell antigen Leu2|T-cell surface glycoprotein CD8 alpha chain|T-lymphocyte differentiation antigen T8/Leu-2|T8 T-cell ant
-
Interactome
-
Pathway
- +1-909-264-1399
+1-909-992-0619
Toll Free : +1-877-853-6098 - +1-909-992-3401
- sales@abnova.com