TNFSF8 Tetramer (Human) Recombinant Protein
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD30 Ligand, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD30 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human TNFSF8 (P32971, Gln63-Asp234) partial recombinant protein with His tag at N-terminus expressed in HEK293 cells.
Sequence
Gln63-Asp234
Host
Human
Theoretical MW (kDa)
20.7
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 8.9 ng/mL, messured by ELISA at 5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human CD30 Ligand is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human CD30 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human CD30 Ligand, His Tag at 5 ug/mL (100 uL/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 8.9 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — TNFSF8
Entrez GeneID
944Protein Accession#
P32971Gene Name
TNFSF8
Gene Alias
CD153, CD30L, CD30LG, MGC138144
Gene Description
tumor necrosis factor (ligand) superfamily, member 8
Omim ID
603875Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. [provided by RefSeq
Other Designations
CD153 antigen|CD30 antigen ligand|CD30 ligand|OTTHUMP00000022762
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Interactome
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Pathway
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Disease
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