OSMR | IL6ST (Human) Recombinant Protein
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More Files
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Enzyme-linked Immunoabsorbent Assay
Immobilized Human OSMR&GP130, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 7.0 ng/mL determined by ELISA.
SEC-HPLC
The purity of Human OSMR&GP130 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human OSMR&GP130 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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Specification
Product Description
Human OSMR | IL6ST (Q99650-1 | P40189-1, Glu28 - Met740 & Glu23 - Glu619) partial recombinant protein with His tag and Flag tag at C-terminus expressed in HEK293 cells.
Sequence
Glu28 - Met740 & Glu23 - Glu619
Host
Human
Theoretical MW (kDa)
82.2, 68.83
Form
Lyophilized
Preparation Method
Mammalian cell (Expi293, high-yield transient HEK293) expression system
Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
The EC50 was 7.0 ng/mL, messured by ELISA at 0.5 ug/mL.
Quality Control Testing
SEC-HPLC and Tris-Bis PAGE
SEC-HPLC
The purity of Human OSMR&GP130 is greater than 95% as determined by SEC-HPLC.
Tris-Bis PAGE
Human OSMR&GP130 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Recommend Usage
Biological Activity
ELISA
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from sterile distilled Water is > 100 ug/mL
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Human OSMR&GP130, His Tag at 0.5 ug/mL (100 uL/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 7.0 ng/mL determined by ELISA.Functional Study
SDS-PAGE
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Gene Info — IL6ST
Entrez GeneID
3572Protein Accession#
Q99650-1 | P40189-1Gene Name
IL6ST
Gene Alias
CD130, CDw130, GP130, GP130-RAPS, IL6R-beta
Gene Description
interleukin 6 signal transducer (gp130, oncostatin M receptor)
Omim ID
600694Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
CD130 antigen|IL6ST nirs variant 3|OTTHUMP00000122545|gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form|gp130 transducer chain|interleukin 6 signal transducer|interleukin receptor beta chain|membrane glycoprotein gp130|oncostatin M
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Gene Info — OSMR
Entrez GeneID
9180Protein Accession#
Q99650-1 | P40189-1Gene Name
OSMR
Gene Alias
MGC150626, MGC150627, MGC75127, OSMRB
Gene Description
oncostatin M receptor
Omim ID
601743Gene Ontology
HyperlinkGene Summary
Oncostatin M is a member of the IL6 family of cytokines. Functional receptors for IL6 family cytokines are multisubunit complexes involving members of the hematopoietin receptor superfamily. Many IL6 cytokines utilize gp130 as a common receptor subunit. OSM binds to the gp130 receptor subunit and, in association with the leukemia inhibitory factor receptor, induces a proliferative response in permissive cells. OSMR is an alternative subunit (for an OSM receptor complex (a heterodimer of gp130 and OSMR) that is activated by OSM but not by LIF [provided by RefSeq
Other Designations
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