EPO (Human) Recombinant Protein
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Result of bioactivity analysis
Result of bioactivity analysis
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Specification
Product Description
Human EPO (P01588, 28 a.a. - 193 a.a.) partial length recombinant protein with His tag expressed in Baculovirus expression system.
Sequence
APPRLICDSRVLERYLLEAKEAENITTGCAEHCSLNENITVPDTKVNFYAWKRMEVGQQAVEVWQGLALLSEAVLRGQALLVNSSQPWEPLQLHVDKAVSGLRSLTTLLRALGAQKEAISPPDAASAAPLRTITADTFRKLFRVYSNFLRGKLKLYTGEACRTGDR
Host
Viruses
Theoretical MW (kDa)
19.5
Form
Liquid
Preparation Method
Baculovirus expression system
Purity
> 95% by SDS-PAGE
Endotoxin Level
< 1 EU per 1 ug of protein (determined by LAL method)
Activity
ED50 is < 0.5 ng/mL, measured in a cell proliferation assay using TF-1 human erythroleukemic cells.
Quality Control Testing
3 ug by SDS-PAGE under reducing condition and visualized by Coomassie blue stain.
Storage Buffer
In Phosphate-Buffer Saline pH 7.4 (10% glycerol)
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — EPO
Entrez GeneID
2056Protein Accession#
P01588Gene Name
EPO
Gene Alias
EP, MGC138142
Gene Description
erythropoietin
Omim ID
133170Gene Ontology
HyperlinkGene Summary
This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. [provided by RefSeq
Other Designations
epoetin
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Interactome
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Pathway
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Disease
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