FASLG (Human) Recombinant Protein
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Specification
Product Description
Human FASLG recombinant protein with polyhistidine-SUMO tag at the N-terminus expressed in Escherichia coli.
Sequence
QIGHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQSCNNLPLSHKVYMRNSKYPQDLVMMEGKMMSYCTTGQMWARSSYLGAVFNLTSADHLYVNVSELSLVNFEESQTFFGLYKL with polyhistidine tag and sumo tag at the N-terminus.
Host
Escherichia coli
Specificity
23
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 98% as determined by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ ug of protein by the LAL method.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue.
SDS-PAGE analysis of FASLG (Human) Recombinant Protein.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from a solution containing 1X PBS, pH 8.0. Reconstitute the lyophilized powder in ddH2O to a concentration not less than 100 ug/mL.
Storage Instruction
Lyophilized protein should be stored at -20°C. Protein aliquots should be stored at-20°C to -80°C. This product is stable for one year.
Avoid repeated freeze/thaw cycles.Note
Result of activity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — FASLG
Entrez GeneID
356Gene Name
FASLG
Gene Alias
APT1LG1, CD178, CD95L, FASL, TNFSF6
Gene Description
Fas ligand (TNF superfamily, member 6)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is the ligand for FAS. Both are transmembrane proteins. Interaction of FAS with this ligand is critical in triggering apoptosis of some types of cells such as lymphocytes. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). [provided by RefSeq
Other Designations
CD95 ligand|OTTHUMP00000032708|apoptosis (APO-1) antigen ligand 1|fas ligand|tumor necrosis factor (ligand) superfamily, member 6
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