CD28 (Human) Recombinant Protein
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Specification
Product Description
Human CD28 (P10747, 19 a.a. - 152 a.a.) partial recombinant protein with hFc tag at C-terminus expressed in HEK293 cell.
Sequence
NKILVKQSPMLVAYDNAVNLSCKYSYNLFSREFRASLHKGLDSAVEVCVVYGNYSQQLQVYSKTGFNCDGKLGNESVTFYLQNLYVNQTDIYFCKIEVMYPPPYLDNEKSNGTIIHVKGKHLCPSPLFPGPSKP
Host
Mammals
Theoretical MW (kDa)
66-70
Form
Lyophilized
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.2 EU per 1 ug of protein (determined by gel clotting method)
Activity
Immobilized CD28, hFc, Human at 2.0 ug/mL (100 uL/well) can bind human Biotin-B7-1 (CD80) Fc when detected by Streptavidin-HRP second antibody.
Storage Buffer
Lyophilized from sterile distilled Water up to 100 ug/mL
Storage Instruction
Store at 4°C to 8°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
Functional Study
SDS-PAGE
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Gene Info — CD28
Entrez GeneID
940Protein Accession#
P10747Gene Name
CD28
Gene Alias
MGC138290, Tp44
Gene Description
CD28 molecule
Omim ID
186760Gene Ontology
HyperlinkGene Summary
CD28 costimulation is essential for CD4 (MIM 186940)-positive T-cell proliferation, survival, interleukin-2 (IL2; MIM 147680) production, and T-helper type-2 (Th2) development.[supplied by OMIM
Other Designations
CD28 antigen|CD28 antigen (Tp44)|T-cell-specific surface glycoprotein
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Interactome
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Pathway
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Disease
- Acute Disease
- Arthritis
- Asthma
- Behcet Syndrome
- Bronchiolitis
- Carcinoma
- Celiac Disease
- Cholangitis
- Colon cancer
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