Lep (Rat) Recombinant Protein
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Specification
Product Description
Rat Lep (P50596, 22 a.a. - 167 a.a.) partial recombinant protein with an N-terminal Met expressed in Escherichia coli.
Sequence
VPIHKVQDDTKTLIKTIVTRINDISHTQSVSARQRVTGLDFIPGLHPILSLSKMDQTLAVYQQILTSLPSQNVLQIAHDLENLRDLLHLLAFSKSCSLPQTRGLQKPESLDGVLEASLYSTEVVALSRLQGSLQDILQQLDLSPEC
Host
Escherichia coli
Theoretical MW (kDa)
~ 16.3
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purity
> 95% as analyzed by SDS-PAGE.
> 95% as analyzed by HPLC.Endotoxin Level
< 0.2 EU/ ug of protein (gel clotting method)
Activity
ED50 < 10.0 ug/mL, measured by a cell proliferation assay using LoVo cells, corresponding to a specific activity of > 100.0 units/mg.
Recommend Usage
Biological Activity
SDS-PAGE
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0. Reconstitute the lyophilized powder in ddH2O up to 100 ug/mL.
Storage Instruction
Store at 4°C for 1 week. For long term storage store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
SDS-PAGE
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Gene Info — Lep
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