IL15 (Human) Recombinant Protein
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Specification
Product Description
Human IL15 recombinant protein with polyhistidine tag at the N-terminus expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
Calculated MW: 13.7
Form
Lyophilized
Preparation Method
Escherichia coli expression system
Purification
Ni-NTA chromatography
Purity
> 97% by SDS-PAGE
Endotoxin Level
< 0.05 EU/ug
Activity
The ED50 for this effect is < 3 ng/mL, measured by the induction of proliferation in CTLL-2 cells. The specific activity of recombinant human IL-15 is > 2 x 106 IU/mg, which is calibrated against the human IL-15 WHO International Standard (NIBSC code: 95/554).
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
Recommend Usage
SDS-PAGE
The optimal working dilution should be determined by the end user.Regulatory Status
GMP grade
Storage Buffer
Lyophilized from NaPi buffer, 0.018% SDS, pH 7.5.
Reconstitute the lyophilized protein in sterile H2O to a concentration of at least 0.5 mg/mL and incubate the stock solution for at least 20 min to ensure sufficient redissolution. Please use the protein within one month after reconstitution.Storage Instruction
Store at -20°C for 12 months in lyophilized state.
After reconstitution with deionized water, store at -20 or -80°C for 1 month.
Aliquot to avoid repeated freezing and thawing. -
Applications
Functional Study
The ED50 for this effect is 0.5-3 ng/mL, measured by the induction of MO7e human megakaryocytic leukemic proliferation.SDS-PAGE
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Gene Info — IL15
Entrez GeneID
3600Gene Name
IL15
Gene Alias
IL-15, MGC9721
Gene Description
interleukin 15
Omim ID
600554Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000164617
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