E (SARS-CoV-2) Recombinant Protein
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Specification
Product Description
SARS-CoV-2 E (QHD43418.1, 1 a.a. - 75 a.a.) recombinant protein with an initial Met, His-Avi tag at N-terminal expressed in Escherichia coli.
Host
Escherichia coli
Theoretical MW (kDa)
11.4
Form
Liquid
Preparation Method
Escherichia coli expression system
Purification
Affinity purification
Purity
> 95% by SDS-PAGE.
Endotoxin Level
< 0.1 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized E (SARS-CoV-2) Recombinant Protein at 2 ug/mL (100 uL/well) can bind Recombinant 2019-nCoV Nucleocapsid with a linear range of 1.2-41.1 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 12 kDa.
Storage Buffer
In 20 mM Tris, 250 mM NaCl, pH 8.0 (0.5% TritonX-100).
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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