S (R682G/R683S/R685S/K986P/V987P) (SARS-CoV-2) ECD Recombinant Protein
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Specification
Product Description
SARS-CoV-2 S ECD (QHD43416.1, 15 a.a. - 1208 a.a.) R682G/R683S/R685S/K986P/V987P mutant partial recombinant protein with His tag at C-terminal expressed in HEK293 cells.
Host
Human
Theoretical MW (kDa)
132.9
Form
Liquid
Preparation Method
Mammalian cell (HEK293) expression system
Purification
Affinity purification
Purity
> 97% by SDS-PAGE.
Endotoxin Level
< 1.0 EU/ug of the protein by LAL method.
Activity
Measured by the binding ability in a functional ELISA. Immobilized S (SARS-CoV-2) ECD Recombinant Protein at 2 ug/mL (100 uL/well) can bind recombinant human ACE2 with a linear range of 1.5-35.1 ng/mL.
Quality Control Testing
SDS-PAGE Stained with Coomassie Blue
This protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 170-220 kDa.
Storage Buffer
In PBS, pH 7.4.
Storage Instruction
Store at -80°C.
Aliquot to avoid repeated freezing and thawing.Note
Result of bioactivity analysis
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Applications
SDS-PAGE
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