DNASE1 Recombinant Protein
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Specification
Product Description
Solution. Animal derived components free. RNases & proteases free. DNASE1 (Bovine) Recombinant Protein is expressed in Pichia pastoris.
Biological function
Bovine pancreatic deoxyribonuclease is an endonuclease which splits phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide yielding polynucleotides with free hydroxyl group at the 3' position and phosphate group at the 5' position.
Host
Yeast
Theoretical MW (kDa)
31.3
Form
Liquid
Preparation Method
Yeast expression system
Activity
>=2 units per ml. One Unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C when acting upon highly polymerized DNA at pH 5.0.
Recommend Usage
Deoxyribonuclease I Assay; Removing genomic DNA from RNA preparations prior to RT-PCR; Degradation of DNA templates after transcription reactions; Removing unwanted DNA from samples prior to Northern blotting; Removing DNA during biopharma and bioprocessing procedures.
Supplied Product
10x reaction buffer
Storage Buffer
In 5 mM Calcium Acetate, 4 mg/ml glycine (stabilizer), pH 5.0 and 50% glycerol
Storage Instruction
Store at -20°C.
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Applications
Enzyme Activity
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Gene Info — DNASE1
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