NECTIN3 (Human) Recombinant Protein (Biotin)
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More Files
- More Functions
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human Nectin-3, His Tag at 2 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.2 ng/mL determined by ELISA (QC Test).
Surface Plasmon Resonance
Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200).
Tris-Bis PAGE
Biotinylated Human NECTIN3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
SEC-HPLC
The purity of Biotinylated Human NECTIN3 is greater than 95% as determined by SEC-HPLC.
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Specifications
Product Description
Human NECTIN3 (Q9NQS3-1, Gly58-Asp400) partial recombinant protein with His-Avi tag at the C-Terminus expressed in HEK293 cells.
Sequence
Gly58-Asp400
Host
Human
Theoretical MW (kDa)
40.6
Reactivity
Human
Form
Lyophilized
Conjugation
Biotin
Preparation Method
Mammalian cell (HEK293) expression system
Purity
> 95% by Tris-Bis PAGE
> 95% by HPLCEndotoxin Level
< 0.1 EU per 1 ug as determined by the LAL method.
Activity
The EC50 was 3.2 ng/mL, measured by ELISA at 2 ug/mL. The affinity constant was 0.13 uM, determined by SPR assay.
Recommend Usage
Biological Activity
ELISA
Tris-Bis PAGE
SEC-HPLC
SPR
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 0.22 um filtered solution in PBS, 250 mM NaCl, pH 7.4. (8% trehalose).
Storage Instruction
Store at -20°C for 12 months.
After reconstitution, store at 4°C for 2-7 days, or store at -80°C for 3-6 months.
Aliquot to avoid repeated freezing and thawing.Note
Please use within one month after protein reconstitution.
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Applications
Enzyme-linked Immunoabsorbent Assay
Immobilized Biotinylated Human Nectin-3, His Tag at 2 ug/mL (100 uL/well) on the streptavidin precoated plate (5 ug/mL). Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 3.2 ng/mL determined by ELISA (QC Test).Functional Study
Surface Plasmon Resonance
Biotinylated Human Nectin-3, His Tag immobilized on CM5 Chip can bind Human Nectin-1, His Tag with an affinity constant of 0.13 uM as determined in SPR assay (Biacore T200). -
Gene Info — PVRL3
Entrez GeneID
25945Protein Accession#
Q9NQS3-1Gene Name
PVRL3
Gene Alias
CD113, CDw113, DKFZp566B0846, FLJ90624, PPR3, PRR3, PVRR3, nectin-3
Gene Description
poliovirus receptor-related 3
Omim ID
607147Gene Ontology
HyperlinkGene Summary
Nectins (e.g., PVRL1; MIM 600644) are immunoglobulin-like adhesion molecules that interact with afadin (AF6; MIM 159559). Afadin is an actin filament-binding protein that connects nectins to the actin cytoskeleton. The nectin-afadin system organizes adherens junctions cooperatively with the cadherin (see MIM 192090)-catenin (see MIM 116805) system in epithelial cells.[supplied by OMIM
Other Designations
nectin 3
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Interactomes
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Pathways
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Diseases
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