BAK1 monoclonal antibody, clone AT38E2
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More Files
- More Functions
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: A549 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: MCF7 cell lysate, Lane 7: PC3 cell lysate.
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and BAK1 monoclonal antibody, clone AT38E2 (Cat # MAB8753) (1 : 500) with Texas Red (Red).
Flow Cytometry
Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant BAK1.
Immunogen
Recombinant protein corresponding to amino acids 29-187 of human BAK1.
Host
Mouse
Reactivity
Human, Mouse
Form
Liquid
Purification
Protein G purification
Concentration
1 mg/mL
Isotype
IgG2a, kappa
Recommend Usage
ELISA
Flow Cytometry
Immunocytochemistry
Immunofluorescence
Western Blot
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4 (10% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western blot analysis of Lane 1: 293T cell lysate, Lane 2: HeLa cell lysate, Lane 3: A431 cell lysate, Lane 4: A549 cell lysate, Lane 5: Jurkat cell lysate, Lane 6: MCF7 cell lysate, Lane 7: PC3 cell lysate.Immunocytochemistry
Immunofluorescence
Immunofluorescence of human HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and BAK1 monoclonal antibody, clone AT38E2 (Cat # MAB8753) (1 : 500) with Texas Red (Red).Enzyme-linked Immunoabsorbent Assay
Flow Cytometry
Flow cytometry analysis of HeLa cell line, staining at 2-5 ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line). -
Gene Info — BAK1
Entrez GeneID
578Protein Accession#
NP_001179Gene Name
BAK1
Gene Alias
BAK, BAK-LIKE, BCL2L7, CDN1, MGC117255, MGC3887
Gene Description
BCL2-antagonist/killer 1
Omim ID
600516Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form oligomers or heterodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. This protein also interacts with the tumor suppressor P53 after exposure to cell stress. [provided by RefSeq
Other Designations
BCL2-like 7 protein|Bcl-2 homologous antagonist/killer|OTTHUMP00000016211|OTTHUMP00000039620|apoptosis regulator BAK|pro-apoptotic protein BAK
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Interactome
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Disease
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