CD72 monoclonal antibody, clone 3F3 (PE)
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Specification
Product Description
Mouse monoclonal antibody raised against native CD72.
Immunogen
Native purified CD72 from normal human lymphocytes from lymph node.
Host
Mouse
Theoretical MW (kDa)
39-43
Reactivity
Human
Specificity
This antibody 3F3 reacts with CD72, a 39-43 KDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
Form
Liquid
Conjugation
PE
Isotype
IgG2b
Recommend Usage
Flow Cytometry (20 ul in human blood cells 100 ul in whole blood or 106 cells in a suspension)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.2% BSA, 0.09% sodium azide)
Storage Instruction
Store in the dark at 4°C. Do not freeze.
Avoid prolonged exposure to light.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Immunoprecipitation
Flow Cytometry
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Gene Info — CD72
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Interactome
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Pathway
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Disease
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Publication Reference
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CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O.
PLoS One 2007 Sep; 2(9):e818.
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Requirement for CD100-CD72 interactions in fine-tuning of B-cell antigen receptor signaling and homeostatic maintenance of the B-cell compartment.
Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H.
International Immunology 2005 Oct; 17(10):1277.
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The CD100-CD72 interaction: a novel mechanism of immune regulation.
Kumanogoh A, Kikutani H.
Trends Immunol 2001 Dec; 22(12):670.
Application:Flow Cyt, Human, Immune cells.
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CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72.
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