AP2A1 monoclonal antibody, clone AFDO-1
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of A-431 cell lysate.
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human AP2A1.
Immunogen
A synthetic peptide corresponding to human AP2A1.
Host
Rabbit
Reactivity
Human
Specificity
The antibody reacts with human AP2A1, in native form and recombinant. Superfamily members of AP2A1 are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Flow Cytometry (1:100)
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of A-431 cell lysate.Immunohistochemistry
Immunocytochemistry
Immunofluorescence
Flow Cytometry
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Gene Info — AP2A1
Entrez GeneID
160Protein Accession#
O95782Gene Name
AP2A1
Gene Alias
ADTAA, AP2-ALPHA, CLAPA1
Gene Description
adaptor-related protein complex 2, alpha 1 subunit
Omim ID
601026Gene Ontology
HyperlinkGene Summary
This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq
Other Designations
100 kDa coated vesicle protein A|adaptin, alpha A|clathrin-associated/assembly/adaptor protein, large, alpha 1
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Interactome
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Pathway
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