HIST1H1C monoclonal antibody, clone AEDF-8
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human HIST1H1C.
Immunogen
A synthetic peptide corresponding to human HIST1H1C.
Host
Rabbit
Reactivity
Human, Mouse, Rat
Specificity
The antibody reacts with human, mouse, rat HIST1H1C, in native form and recombinant. Superfamily members of HIST1H1C are not reactive to this antibody.
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C for short term storage. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of Lane 1: MCF7 cell lysate; Lane 2: NIH/3T3 cell lysate; Lane 3: C6 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of mouse colon tissue.Immunocytochemistry
Immunocytochemistry staining of Hela cells (green), the nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.Immunofluorescence
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Gene Info — HIST1H1C
Entrez GeneID
3006Protein Accession#
P16403Gene Name
HIST1H1C
Gene Alias
H1.2, H1F2, MGC3992
Gene Description
histone cluster 1, H1c
Omim ID
142710Gene Ontology
HyperlinkGene Summary
Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq
Other Designations
H1 histone family, member 2|OTTHUMP00000017749|histone 1, H1c|histone H1d
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