KLK3 monoclonal antibody, clone RM323
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More Files
- More Functions
Western Blot
Western Blot analysis of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non reduced (-) or reduced (+) conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate cancer.
Enzyme-linked Immunoabsorbent Assay
A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of KLK3 monoclonal antibody, clone RM323 (Cat# MAB21979) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.
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Specification
Product Description
Rabbit recombinant monoclonal antibody raised against human KLK3.
Antibody Species
Rabbit
Immunogen
Original antibody is raised against native purified KLK3 from human seminal fluid.
Reactivity
Human
Specificity
This antibody reacts to native human PSA (Prostate Specific Antigen), including both free (unbound) form and in a complex with ACT (alpha 1-antichymotrypsin). It may react to reduced PSA at a higher concentration.
Form
Liquid
Purification
Protein A purification
Isotype
IgG
Recommend Usage
ELISA (1:1000-5000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1000)
Western Blot (1:1000-2500)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)
Storage Instruction
Store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of PSA (purified from human seminal fluid), ACT (alpha 1-antichymotrypsin, from human plasma), and PSA-ACT complex, under non reduced (-) or reduced (+) conditions.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of human prostate cancer.Enzyme-linked Immunoabsorbent Assay
A titer ELISA of PSA (purified from human seminal fluid). The plate was coated with different amounts of PSA. A serial dilution of KLK3 monoclonal antibody, clone RM323 (Cat# MAB21979) was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.Enzyme-linked Immunoabsorbent Assay
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Gene Info — KLK3
Entrez GeneID
354Gene Name
KLK3
Gene Alias
APS, KLK2A1, PSA, hK3
Gene Description
kallikrein-related peptidase 3
Omim ID
176820Gene Ontology
HyperlinkGene Summary
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates several transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
P-30 antigen|gamma-seminoprotein|kallikrein 3, (prostate specific antigen)|prostate specific antigen|semenogelase|seminin
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Interactome
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Pathway
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Disease
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