PCK2 monoclonal antibody, clone 3D3D9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: PCK2-hIgGFc transfected HEK293 cell lysates with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach carcinoma with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).
Flow Cytometry
Flow cytometric analysis of HeLa cells with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474) (Green). Red: Negative Control.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PCK2.
Immunogen
Recombinant protein corresponding to amino acids 44-175 of human PCK2.
Host
Mouse
Theoretical MW (kDa)
70.7
Reactivity
Human
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-1:400)
Immunocytochemistry (1:200-1:1000)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1:1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of Lane 1: HEK293 and Lane 2: PCK2-hIgGFc transfected HEK293 cell lysates with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human stomach carcinoma with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).Immunocytochemistry
Enzyme-linked Immunoabsorbent Assay
ELISA analysis with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474).Flow Cytometry
Flow cytometric analysis of HeLa cells with PCK2 monoclonal antibody, clone 3D3D9 (Cat # MAB21474) (Green). Red: Negative Control. -
Gene Info — PCK2
Entrez GeneID
5106Protein Accession#
Q16822Gene Name
PCK2
Gene Alias
PEPCK, PEPCK-M, PEPCK2
Gene Description
phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Omim ID
261650Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq
Other Designations
OTTHUMP00000164700|PEP carboxykinase|mitochondrial phosphoenolpyruvate carboxykinase 2|phosphoenolpyruvate carboxylase|phosphopyruvate carboxylase
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Interactome
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Pathway
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Disease
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