ATG12 monoclonal antibody, clone ABID-1
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Specification
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ATG12.
Immunogen
A synthetic peptide corresponding to human ATG12.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunocytochemistry (1:50-1:200)
Immunofluorescence (1:50-1:200)
Immunohistochemistry (1:50-1:200)
Immunoprecipitation (1:50)
Flow Cytometry (1:100)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of 293 cell lysate with ATG12 monoclonal antibody.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunocytochemistry
Immunofluorescence
Immunoprecipitation
Flow Cytometry
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Gene Info — ATG12
Entrez GeneID
9140Protein Accession#
O94817Gene Name
ATG12
Gene Alias
APG12, APG12L, FBR93, HAPG12
Gene Description
ATG12 autophagy related 12 homolog (S. cerevisiae)
Omim ID
609608Gene Ontology
HyperlinkGene Summary
Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM
Other Designations
APG12 autophagy 12-like|Apg12 (autophagy, yeast) homolog
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Interactome
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Pathway
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Disease
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