ATG10 monoclonal antibody, clone ABBD-1
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Specifications
Product Description
Rabbit monoclonal antibody raised against synthetic peptide of human ATG10.
Immunogen
A synthetic peptide corresponding to human ATG10.
Host
Rabbit
Reactivity
Human
Form
Liquid
Purification
Affinity purification
Isotype
IgG
Recommend Usage
Immunoprecipitation (1:50)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Instruction
Store at -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of HeLa cell lysate with ATG10 monoclonal antibody.Immunoprecipitation
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Gene Info — ATG10
Entrez GeneID
83734Protein Accession#
Q9H0Y0Gene Name
ATG10
Gene Alias
APG10, APG10L, DKFZp586I0418, FLJ13954, pp12616
Gene Description
ATG10 autophagy related 10 homolog (S. cerevisiae)
Omim ID
610800Gene Ontology
HyperlinkGene Summary
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM
Other Designations
APG10 autophagy 10-like
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Interactomes
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Diseases
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