AP2M1 monoclonal antibody, clone 4F1B2
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More Files
- More Functions
Western Blot (Cell lysate)
Western blot analysis of (1) COS7 cell, (2) SK-Br-3 cell, (3) MCF-7 cell, (4) T47D cell, (5) HEK293 cell with AP2M1 monoclonal antibody.
Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AP2M1 monoclonal antibody, clone 4F1B2.
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human AP2M1.
Immunogen
Recombinant protein corresponding to amino acid 298-435 of human AP2M1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
49.7
Reactivity
Human, Monkey
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:300-1:800)
Immunocytochemistry
Flow Cytometry
Immunohistochemistry
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western blot analysis of (1) COS7 cell, (2) SK-Br-3 cell, (3) MCF-7 cell, (4) T47D cell, (5) HEK293 cell with AP2M1 monoclonal antibody.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of AP2M1 monoclonal antibody, clone 4F1B2. -
Gene Info — AP2M1
Entrez GeneID
1173Gene Name
AP2M1
Gene Alias
AP50, CLAPM1, mu2
Gene Description
adaptor-related protein complex 2, mu 1 subunit
Omim ID
601024Gene Ontology
HyperlinkGene Summary
This gene encodes a subunit of the heterotetrameric coat assembly protein complex 2 (AP2), which belongs to the adaptor complexes medium subunits family. The encoded protein is required for the activity of a vacuolar ATPase, which is responsible for proton pumping occurring in the acidification of endosomes and lysosomes. The encoded protein may also play an important role in regulating the intracellular trafficking and function of CTLA-4 protein. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
AP-2 mu 2 chain|HA2 50 kDA subunit|clathrin adaptor complex AP2, mu subunit|clathrin assembly protein complex 2 medium chain|clathrin coat adaptor protein AP50|clathrin-associated/assembly/adaptor protein, medium 1|plasma membrane adaptor AP-2 50kDA prote
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Interactome
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Pathway
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Disease
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