BIN1 monoclonal antibody, clone 3B6F10
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More Files
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Specification
Product Description
Mouse monoclonal antibody raised against recombinant human BIN1.
Immunogen
Recombinant protein corresponding to amino acids 189-398 of of human BIN1 from E. coli.
Host
Mouse
Theoretical MW (kDa)
64.7
Reactivity
Human, Mouse
Form
Liquid
Isotype
IgG2b
Recommend Usage
ELISA (1:10000)
Flow Cytometry (1:200-400)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:200-1000)
Western Blot (1:500-1:2000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.05% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Western Blot analysis of human BIN1 recombinant protein.Western Blot
Western Blot analysis of (1) C2C12, (2) A431, (3) HEK293, and (4) MCF-7 cell lysate.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining of cervical cancer tissues with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA analysis of BIN1 monoclonal antibody.Flow Cytometry
Flow cytometric analysis of HeLa cells using BIN1 mouse monoclonal antibody (green) and negative control (red). -
Gene Info — BIN1
Entrez GeneID
274Gene Name
BIN1
Gene Alias
AMPH2, AMPHL, DKFZp547F068, MGC10367, SH3P9
Gene Description
bridging integrator 1
Gene Ontology
HyperlinkGene Summary
This gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described. [provided by RefSeq
Other Designations
OTTHUMP00000162179|OTTHUMP00000162183|amphiphysin II|amphiphysin-like|box dependant MYC interacting protein 1
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Interactome
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Disease
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