PHGDH monoclonal antibody, clone CL0555
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with PHGDH monoclonal antibody, clone CL0555 (Cat # MAB15639).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with PHGDH monoclonal antibody, clone CL0555 (Cat # MAB15639) shows moderate cytoplasmic immunoreactivity in tumor cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human PHGDH.
Immunogen
Recombinant protein corresponding to human PHGDH.
Sequence
LEEIWPLCDFITVHTPLLPSTTGLLNDNTFAQCKKGVRVVNCARGGIVDEGALLRALQSGQCAGAALDVFTEEPPRDRALVDHENVISCPHLGASTKEAQSRCGEEIAVQFVDM
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:500-1:1000)
Western Blot (1:500-1:1000)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of RT-4 cell lysate with PHGDH monoclonal antibody, clone CL0555 (Cat # MAB15639).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human breast cancer with PHGDH monoclonal antibody, clone CL0555 (Cat # MAB15639) shows moderate cytoplasmic immunoreactivity in tumor cells. -
Gene Info — PHGDH
Entrez GeneID
26227Protein Accession#
O43175Gene Name
PHGDH
Gene Alias
3-PGDH, 3PGDH, MGC3017, PDG, PGAD, PGD, PGDH, SERA
Gene Description
phosphoglycerate dehydrogenase
Gene Ontology
HyperlinkGene Summary
3-Phosphoglycerate dehydrogenase (PHGDH; EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM
Other Designations
3-phosphoglycerate dehydrogenase|OTTHUMP00000013926
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Interactome
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Pathway
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Disease
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