NES monoclonal antibody, clone CL0197
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More Files
- More Functions
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with NES monoclonal antibody, clone CL0197 (Cat # MAB15557).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with NES monoclonal antibody, clone CL0197 (Cat # MAB15557) shows strong immunoreactivity in the endothelial cells.
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Specification
Product Description
Mouse monoclonal antibody raised against partial recombinant human NES.
Immunogen
Recombinant protein corresponding to human NES.
Epitope
This antibody binds to an epitope located within the peptide sequence VGGLGDPGHL as determined by overlapping synthetic peptides.
Sequence
DPEGQSQQVGAPGLQAPQGLPEAIEPLVEDDVAPGGDQASPEVMLGSEPAMGESAAGAEPGPGQGVGGLGDPGHLTREEVMEPPLEEESLEAKRVQGLEGPRKDLEEAGGLGTEFSELP
Host
Mouse
Reactivity
Human
Form
Liquid
Purification
Protein A purification
Isotype
IgG1
Recommend Usage
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:2500-1:5000)
Western Blot (1:100-1:250)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS, pH 7.2 (40% glycerol, 0.02% sodium azide).
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot (Cell lysate)
Western Blot analysis of U-251 MG cell lysate with NES monoclonal antibody, clone CL0197 (Cat # MAB15557).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human cerebral cortex with NES monoclonal antibody, clone CL0197 (Cat # MAB15557) shows strong immunoreactivity in the endothelial cells. -
Gene Info — NES
Entrez GeneID
10763Protein Accession#
P48681Gene Name
NES
Gene Alias
FLJ21841, Nbla00170
Gene Description
nestin
Omim ID
600915Gene Ontology
HyperlinkGene Summary
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM
Other Designations
OTTHUMP00000032198
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Interactome
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Disease
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