Actinin-alpha monoclonal antibody, clone EA-53
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Specifications
Product Description
Mouse monoclonal antibody raised against native actinin-alpha.
Immunogen
Native purified rabbit actinin-alpha.
Host
Mouse
Theoretical MW (kDa)
100
Reactivity
Human, Mouse, Rabbit, Rat
Form
Lyophilized
Purification
Affinity purification
Isotype
IgG1
Recommend Usage
Western Blot (3.5 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (3.5-7 ug/mL)
Immunohistochemistry (Frozen sections) (3.57 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
Lyophilized from 1.2% sodium acetate (2 mg BSA, 0.01 mg sodium azide)
Storage Instruction
Store at -20°C on dry atmosphere.
After reconstitution with 1 mL of 1.2% sodium acetate or neutral PBS and concentration will be 100 ug/mL, store at -20°C or lower.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Applications
Western Blot
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
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Publication Reference
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Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.
Youssoufian H, McAfee M, Kwiatkowski DJ.
American Journal of Human Genetics 1990 Jul; 47(1):62.
Application:IF, WB-Tr, Monkey, COS-1 cells.
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Cloning and chromosomal localization of the human cytoskeletal alpha-actinin gene reveals linkage to the beta-spectrin gene.
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