TGFA monoclonal antibody, clone TGFA/1119
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Specification
Product Description
Mouse monoclonal antibody raised against full length recombinant human TGFA.
Immunogen
Recombinant protein corresponding to full length human TGFA.
Host
Mouse
Theoretical MW (kDa)
~6
Reactivity
Human
Specificity
This antibody reacts with the C-terminus of TGFA and shows no cross-reaction with EGF and the neuropeptide synenkephalin.
Form
Liquid
Purification
Protein A/G purification
Isotype
IgG1, kappa
Recommend Usage
Flow Cytometry (0.5-1 ug/million cells in 0.1 mL)
Immunofluorescence (1-2 ug/mL)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (2-4 ug/mL)
The optimal working dilution should be determined by the end user.Storage Buffer
In 10 mM PBS.
Storage Instruction
Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing. -
Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical staining (Formalin-fixed paraffin-embedded sections) of human testicular carcinoma with TGFA monoclonal antibody, clone TGFA/1119 (Cat # MAB14615).Immunofluorescence
Flow Cytometry
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Gene Info — TGFA
Entrez GeneID
7039Protein Accession#
P01135Gene Name
TGFA
Gene Alias
TFGA
Gene Description
transforming growth factor, alpha
Omim ID
190170Gene Ontology
HyperlinkGene Summary
Transforming growth factors (TGFs) are biologically active polypeptides that reversibly confer the transformed phenotype on cultured cells. TGF-alpha shows about 40% sequence homology with epidermal growth factor (EGF; MIM 131530) and competes with EGF for binding to the EGF receptor (MIM 131550), stimulating its phosphorylation and producing a mitogenic response.[supplied by OMIM
Other Designations
transforming growth factor-alpha
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Interactome
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Pathway
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Disease
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Publication Reference
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Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.
Z Bebok, B Markus, P Nemeth.
Breast Cancer Research and Treatment 1994 Jan; 29(3):229.
Application:IHC-Fr, IHC-P, Human, Human breast cancer.
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Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry.
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